Immune compromised zebrafish for optimized cell transplantation

Immune compromised zebrafish for optimized cell transplantation

Immune Compromised Zebrafish

 

Utility of immune compromised animals for efficient transplant engraftment:

Cell transplantation of human and mouse cells into immune compromised mice has greatly impacted our understanding of stem cell function, regeneration following injury, and cancer.  However, these transplantation experiments routinely utilize small cohorts of mice due to high husbandry costs and engraftment of cells is difficult to visualize especially at single cell resolution. Capitalizing on transparent zebrafish and genome engineering approaches, we are developing mutant zebrafish lines that have impaired immune cell function resulting from similar mutations as observed in commonly utilized immunocompromised mouse strains and mimic mutations found in human severe combined immunodeficiency (SCID).  These immune compromised zebrafish lines will be useful for adoptive transfer of zebrafish, mouse, and human cells.  This work is supported by an R24 Resource Grant from the Office of the Director at NIH (R24OD016761).

 

Lines in development:

Lines Developed

Zfin ID

Phenotype

Engraftment

Status

rag2E450fs (AB-strain)

fb101

Absent T cells, reduced B cells

Tumors and Normal Stem Cells

Available

rag2E450fs (casper-strain)

fb101

Absent T cells, reduced B cells

Tumors and Normal Stem Cells

Available

jak3P369fs (casper-strain)

fb102

Absent T and NK cells

Tumors and Normal Stem Cells

Available

prkdcD3612fs (casper-strain)

fb103

Absent T and B cells

Tumors and Normal Stem Cells

Available

Zap70 (AB-strain)

y442

Absent T cells

Tumors and Normal Stem Cells

Available

 

 

 

Non-profit institutions can Order Lines  here.